Journal: Molecular Medicine Reports

Article Title: 1,25-Dihydroxy-Vitamin D3 induces macrophage polarization to M2 by upregulating T-cell Ig-mucin-3 expression

doi: 10.3892/mmr.2019.10047

Figure Lengend Snippet: Effects of Tim-3 gene overexpression on macrophages. The levels of (A) IL-6, (B) IL-10, (C) TNF-α and (D) TGF-β in the control, NC and pTim-3 groups were detected using ELISAs. (E) Tim-3, (F) NOS2 and (G) CD206 protein levels were assessed by western blot analysis. (H) Representative western blot image. (I) Tim-3, (J) NOS2 and (K) CD206 mRNA levels were analyzed by reverse transcription quantitative polymerase chain reaction assays. **P<0.01 vs. control group; ## P<0.01 vs. NC group. Data are presented as mean ± standard deviation (n=5). IL, interleukin; TNF-α, tumor necrosis factor α; TGF-β, transforming growth factor; Tim-3, T-cell Immunoglobulin-mucin-3; NOS2, nitric oxide synthase, inducible; CD206, macrophage mannose receptor 1; pTim-3, overexpressing Tim-3 plasmid.

Article Snippet: The recombinant Tim-3 overexpression plasmid (pTim-3), empty vector (pScramble) and small interfering (si)Tim-3 were purchased from Promega Corporation (Madison, WI, USA).

Techniques: Over Expression, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Plasmid Preparation

Journal: Molecular Medicine Reports

Article Title: 1,25-Dihydroxy-Vitamin D3 induces macrophage polarization to M2 by upregulating T-cell Ig-mucin-3 expression

doi: 10.3892/mmr.2019.10047

Figure Lengend Snippet: Effects of 1,25(OH) 2 D3 on macrophages with different Tim-3 expression levels. RAW264.7 macrophages with silenced Tim-3 expression and Tim-3 overexpression were cultured with 10 −7 M 1,25(OH) 2 D3. ELISAs were used to assess the levels of (A) IL-6, (B) IL-10, (C) TNF-α and (D) TGF-β in the control group. (E) Tim-3, (F) CD206 and (G) NOS2 protein levels were assessed by western blot analysis. (H) Representative western blot image. (I) Tim-3, (J) NOS2 and (K) CD206 mRNA levels were analyzed by reverse transcription quantitative polymerase chain reaction assays. *P<0.05 and **P<0.01 vs. control group; + P<0.05; ++ P<0.01; ## P<0.01; && P<0.01. Data are presented as mean ± standard deviation (n=5). 1,25(OH) 2 D3, 1,25-dihydroxy-vitamin D3; IL, interleukin; TNF-α, tumor necrosis factor α; TGF-β, transforming growth factor; Tim-3, T-cell Immunoglobulin-mucin-3; NOS2, nitric oxide synthase, inducible; CD206, macrophage mannose receptor 1; pTim-3, overexpressing Tim-3 plasmid; si, small interfering.

Article Snippet: The recombinant Tim-3 overexpression plasmid (pTim-3), empty vector (pScramble) and small interfering (si)Tim-3 were purchased from Promega Corporation (Madison, WI, USA).

Techniques: Expressing, Over Expression, Cell Culture, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Plasmid Preparation